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anti scarb1  (Proteintech)


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    Structured Review

    Proteintech anti scarb1
    Anti Scarb1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+scarb1/SR-BI+Antibody/pm41485681-107-24-25
    Average 94 stars, based on 33 article reviews
    anti scarb1 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    other:

    Article Title: The NR_109/FUBP1/c-Myc axis regulates TAM polarization and remodels the tumor microenvironment to promote cancer development
    Article Snippet: The following antibodies were used: anti-c-Myc (Abcam, ab39688, USA), anti-FUBP1 (Proteintech, 24 864–1-AP, China), anti-JTV-1 (Proteintech, 10 424-1-AP, China), anti-Ubiquitin (Abcam, ab134953, USA), anti-CD206 (Abcam, ab64693, USA) and anti-SCARB1 (Proteintech, 21 277–1-AP, China).

    Article Title: Polystyrene nanoplastics disrupted cholesterol/testosterone homeostasis via Smurf1-dependent FTO degradation.
    Article Snippet: Polystyrene nanoplastics (PS-NPs) exposure can induce testosterone decline, but the underlying mechanism remains elusive.. In the present study, prepubertal PS-NPs exposure caused testicular injury and reduced testosterone levels.. RNA sequence analysis indicated that cholesterol homeostasis and PPARα signaling pathways may be involved in the disruption of testosterone biosynthesis.

    Ubiquitin Proteomics:

    Article Title: The NR_109/FUBP1/c-Myc axis regulates TAM polarization and remodels the tumor microenvironment to promote cancer development.
    Article Snippet: .. D ow nloaded from 4 Zhang C, et al. J Immunother Cancer 2023;11:e006230. doi:10.1136/jitc-2022-006230 24 864–1- AP, China), anti- JTV- 1 (Proteintech, 10 424- 1- AP, China), anti- Ubiquitin (Abcam, ab134953, USA), anti- CD206 (Abcam, ab64693, USA) and anti- SCARB1 (Proteintech, 21 277–1- AP, China). .. Protein levels were normalized to the endogenous GAPDH control (Proteintech, 10 494–1- AP, China) and all the antibodies were diluted in Antibody Diluent (Absin, abs954).

    Western Blot:

    Article Title: Huanglian Jiedu decoction inhibits vascular smooth muscle cell-derived foam cell formation by activating autophagy via suppressing P2RY12.
    Article Snippet: Giemsa stain (G1007, Servicebio, Wuhan, China) was used to depict the morphology of VSMCs. .. The following antibodies were used for Western blotting: Anti-PI3 Kinase p110 Beta (20584-1-AP, 1:500, Proteintech, Hubei, China), Anti-SQSTM1 (AF5384, 1:1000, Affinity, Affinity Biosciences, Jiangsu, China; 66184-1-Ig, 1:1000, Proteintech), Anti-SCARB1 (DF6479, 1:1000, Affinity), Anti-Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (4228T, 1:1000, CST), AntiPhospho-AKT (Ser473) (66444-1-Ig, 1:5000, Proteintech), AntiOsteopontin (AF0227, 1:1000, Affinity), Anti-pan-AKT1/2/3 (AF6261, 1:1000, Affinity), Anti-alpha-SMA (AF1032, 1:1000, Affinity), AntiP2RY12 (DF10263, 1:1000, Affinity), Anti-Caspase 3 (AF6311, 1:1000, Affinity), Anti-GAPDH (T0004, 1:5000, Affinity), Anti-TNF alpha (AF7014, 1:500, Affinity), Anti-IL6 (DF6087, 1:1000, Affinity), Anti-SM22 alpha (AF9266, 1:1000, Affinity), Anti-LC3A/B (AF5402, 1:1000, Affinity), and Goat Anti-Rabbit IgG (H + L) (S0001, 1:5000, Affinity). .. For immunofluorescence staining, the sections or cells were incubated with antibodies against LC3A/B (AF5402, 1:100, Affinity), SQSTM1 (66184-1-Ig, 1: 200, Proteintech), α-SMA (AF1032, 1:200, Affinity), PI 3-kinase p110β (sc-376641, 1:200, Santa Cruz, Santa Cruz Biotechnology, CA, USA), and P2RY12 (DF10263, 1:200, Affinity).



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    Image Search Results


    A list of the primers used in the qRT‐PCR.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Huayu Qutan Recipe promotes lipophagy and cholesterol efflux through the mTORC1 / TFEB / ABCA1‐SCARB1 signal axis

    doi: 10.1111/jcmm.18257

    Figure Lengend Snippet: A list of the primers used in the qRT‐PCR.

    Article Snippet: The following primary antibodies were used: Anti‐CD11b (PTM Bio, Cat. PTM‐6088); Anti‐SQSTM1 (Abclonal, Cat.A19700); Anti‐Beclin1 (Cell Signaling Technology, Cat. 3495); Anti‐LC3b (NOVUSBIO, Cat. NB100‐2220); Anti‐LC3A/B (Cell Signaling Technology, Cat. 12741); Anti‐p‐mTOR‐S2448 (Cell Signaling Technology, Cat. 5536); Anti‐mTOR (Cell Signaling Technology, Cat. 2983); Anti‐TFEB (ABBKINE, Cat. ABP56578); Anti‐Phospho‐eIF4EBP1‐S65 Rabbit (Abclonal, Cat.AP1363); Anti‐4EBP1 (Proteintech, Cat. 60246‐1‐Ig); Anti‐P70S6K (Abcam, Cat. ab32529); Anti‐p‐P70S6K (Proteintech, Cat. 14485‐1‐AP); Anti‐ABCA1 (Abclonal, Cat. A7228); Anti‐ABCG1 (Bioss, Cat. bs‐23382R); Anti‐SCARB1 (Bioss, Cat. bs‐23977R).

    Techniques:

    HYQT regulates the mTORC1/TFEB signalling pathway and cholesterol efflux proteins. (A) Detected the proteins expression by WB; (B) p‐mTOR/mTOR; (C) TFEB; (D) Immunofluorescence for TFEB (Scale bar: 50 μm); (E) Ratio of TFEB in nucleus ( n = 12 per group). Normalized ratios of (F) p‐4EBP1/4EBP1, (G) p‐P70S6K/P70S6K, (H) ABCA1 mRNA, (I) ABCA1 protein, (J) ABCG1 mRNA, (K) ABCG1 protein, (L) SCARB1 mRNA, (M) SCARB1 protein. qRT‐PCR and WB data were presented as the mean ± SD ( n = 3). * P < 0.05, ** P < 0.01 versus CTRL, # P < 0.05, ## P < 0.01 versus MOD, △ P < 0.05, △△ P < 0.01 versus HYQT. TFEB proteins were stained with fluorescence (red), and nuclei were stained with DAPI (blue).

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Huayu Qutan Recipe promotes lipophagy and cholesterol efflux through the mTORC1 / TFEB / ABCA1‐SCARB1 signal axis

    doi: 10.1111/jcmm.18257

    Figure Lengend Snippet: HYQT regulates the mTORC1/TFEB signalling pathway and cholesterol efflux proteins. (A) Detected the proteins expression by WB; (B) p‐mTOR/mTOR; (C) TFEB; (D) Immunofluorescence for TFEB (Scale bar: 50 μm); (E) Ratio of TFEB in nucleus ( n = 12 per group). Normalized ratios of (F) p‐4EBP1/4EBP1, (G) p‐P70S6K/P70S6K, (H) ABCA1 mRNA, (I) ABCA1 protein, (J) ABCG1 mRNA, (K) ABCG1 protein, (L) SCARB1 mRNA, (M) SCARB1 protein. qRT‐PCR and WB data were presented as the mean ± SD ( n = 3). * P < 0.05, ** P < 0.01 versus CTRL, # P < 0.05, ## P < 0.01 versus MOD, △ P < 0.05, △△ P < 0.01 versus HYQT. TFEB proteins were stained with fluorescence (red), and nuclei were stained with DAPI (blue).

    Article Snippet: The following primary antibodies were used: Anti‐CD11b (PTM Bio, Cat. PTM‐6088); Anti‐SQSTM1 (Abclonal, Cat.A19700); Anti‐Beclin1 (Cell Signaling Technology, Cat. 3495); Anti‐LC3b (NOVUSBIO, Cat. NB100‐2220); Anti‐LC3A/B (Cell Signaling Technology, Cat. 12741); Anti‐p‐mTOR‐S2448 (Cell Signaling Technology, Cat. 5536); Anti‐mTOR (Cell Signaling Technology, Cat. 2983); Anti‐TFEB (ABBKINE, Cat. ABP56578); Anti‐Phospho‐eIF4EBP1‐S65 Rabbit (Abclonal, Cat.AP1363); Anti‐4EBP1 (Proteintech, Cat. 60246‐1‐Ig); Anti‐P70S6K (Abcam, Cat. ab32529); Anti‐p‐P70S6K (Proteintech, Cat. 14485‐1‐AP); Anti‐ABCA1 (Abclonal, Cat. A7228); Anti‐ABCG1 (Bioss, Cat. bs‐23382R); Anti‐SCARB1 (Bioss, Cat. bs‐23977R).

    Techniques: Expressing, Immunofluorescence, Quantitative RT-PCR, Staining, Fluorescence

    HYQT regulates lipophagy and cholesterol efflux via the mTORC1/TFEB/ABCA1‐SCARB1 signal axis. (A) TFEB, LC3II/I, Beclin1, and p62 proteins expression treated with MHY1485 and DMSO, and (B–E) normalized ratios; (F) Immunofluorescence for TFEB (Scale bar: 50 μm); (G) Ratio of TFEB in nucleus ( n = 12 per group); (H) ABCA1, ABCG1 and SCARB1 proteins expression treated with MHY1485 and DMSO; (I–J) ABCA1, (K,L) ABCG1, (M,N) SCARB1 mRNAs and proteins normalized ratios. qRT‐PCR and WB data were presented as the mean ± SD ( n = 3). * P < 0.05, ** P < 0.01 versus HYQT. TFEB proteins were stained with fluorescence (red), and nuclei were stained with DAPI (blue).

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Huayu Qutan Recipe promotes lipophagy and cholesterol efflux through the mTORC1 / TFEB / ABCA1‐SCARB1 signal axis

    doi: 10.1111/jcmm.18257

    Figure Lengend Snippet: HYQT regulates lipophagy and cholesterol efflux via the mTORC1/TFEB/ABCA1‐SCARB1 signal axis. (A) TFEB, LC3II/I, Beclin1, and p62 proteins expression treated with MHY1485 and DMSO, and (B–E) normalized ratios; (F) Immunofluorescence for TFEB (Scale bar: 50 μm); (G) Ratio of TFEB in nucleus ( n = 12 per group); (H) ABCA1, ABCG1 and SCARB1 proteins expression treated with MHY1485 and DMSO; (I–J) ABCA1, (K,L) ABCG1, (M,N) SCARB1 mRNAs and proteins normalized ratios. qRT‐PCR and WB data were presented as the mean ± SD ( n = 3). * P < 0.05, ** P < 0.01 versus HYQT. TFEB proteins were stained with fluorescence (red), and nuclei were stained with DAPI (blue).

    Article Snippet: The following primary antibodies were used: Anti‐CD11b (PTM Bio, Cat. PTM‐6088); Anti‐SQSTM1 (Abclonal, Cat.A19700); Anti‐Beclin1 (Cell Signaling Technology, Cat. 3495); Anti‐LC3b (NOVUSBIO, Cat. NB100‐2220); Anti‐LC3A/B (Cell Signaling Technology, Cat. 12741); Anti‐p‐mTOR‐S2448 (Cell Signaling Technology, Cat. 5536); Anti‐mTOR (Cell Signaling Technology, Cat. 2983); Anti‐TFEB (ABBKINE, Cat. ABP56578); Anti‐Phospho‐eIF4EBP1‐S65 Rabbit (Abclonal, Cat.AP1363); Anti‐4EBP1 (Proteintech, Cat. 60246‐1‐Ig); Anti‐P70S6K (Abcam, Cat. ab32529); Anti‐p‐P70S6K (Proteintech, Cat. 14485‐1‐AP); Anti‐ABCA1 (Abclonal, Cat. A7228); Anti‐ABCG1 (Bioss, Cat. bs‐23382R); Anti‐SCARB1 (Bioss, Cat. bs‐23977R).

    Techniques: Expressing, Immunofluorescence, Quantitative RT-PCR, Staining, Fluorescence

    Taurine regulates lipid accumulation and mTOR/TFEB/ABCA1‐SCARB1 signal axis. (A) Taurine inhibits lipid droplet accumulation (BODIPY Scale bar: 25 μm, ORO Scale bar: 100 μm). (B,C) Normalized ratios of BODIPY and ORO staining. (D–G) Taurine promotes LC3II/I and Lamp1 proteins. (H–J) Taurine regulates mTOR/TFEB signalling pathway. (K) Taurine regulates cholesterol efflux proteins. (L,M) ABCA1 mRNA and protein, (N,O) ABCG1 mRNA and protein, (P‐Q) SCARB1 mRNA and protein. qRT‐PCR and WB data were presented as the mean ± SD ( n = 3). * P < 0.05, ** P < 0.01 versus CTRL. # P < 0.05, ## P < 0.01 versus MOD, ▲ P < 0.05, ▲▲ P < 0.01 versus taurine (40 mmol/L). ■ P < 0.05, ■■ P < 0.01 versus taurine (80 mmol/L).

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Huayu Qutan Recipe promotes lipophagy and cholesterol efflux through the mTORC1 / TFEB / ABCA1‐SCARB1 signal axis

    doi: 10.1111/jcmm.18257

    Figure Lengend Snippet: Taurine regulates lipid accumulation and mTOR/TFEB/ABCA1‐SCARB1 signal axis. (A) Taurine inhibits lipid droplet accumulation (BODIPY Scale bar: 25 μm, ORO Scale bar: 100 μm). (B,C) Normalized ratios of BODIPY and ORO staining. (D–G) Taurine promotes LC3II/I and Lamp1 proteins. (H–J) Taurine regulates mTOR/TFEB signalling pathway. (K) Taurine regulates cholesterol efflux proteins. (L,M) ABCA1 mRNA and protein, (N,O) ABCG1 mRNA and protein, (P‐Q) SCARB1 mRNA and protein. qRT‐PCR and WB data were presented as the mean ± SD ( n = 3). * P < 0.05, ** P < 0.01 versus CTRL. # P < 0.05, ## P < 0.01 versus MOD, ▲ P < 0.05, ▲▲ P < 0.01 versus taurine (40 mmol/L). ■ P < 0.05, ■■ P < 0.01 versus taurine (80 mmol/L).

    Article Snippet: The following primary antibodies were used: Anti‐CD11b (PTM Bio, Cat. PTM‐6088); Anti‐SQSTM1 (Abclonal, Cat.A19700); Anti‐Beclin1 (Cell Signaling Technology, Cat. 3495); Anti‐LC3b (NOVUSBIO, Cat. NB100‐2220); Anti‐LC3A/B (Cell Signaling Technology, Cat. 12741); Anti‐p‐mTOR‐S2448 (Cell Signaling Technology, Cat. 5536); Anti‐mTOR (Cell Signaling Technology, Cat. 2983); Anti‐TFEB (ABBKINE, Cat. ABP56578); Anti‐Phospho‐eIF4EBP1‐S65 Rabbit (Abclonal, Cat.AP1363); Anti‐4EBP1 (Proteintech, Cat. 60246‐1‐Ig); Anti‐P70S6K (Abcam, Cat. ab32529); Anti‐p‐P70S6K (Proteintech, Cat. 14485‐1‐AP); Anti‐ABCA1 (Abclonal, Cat. A7228); Anti‐ABCG1 (Bioss, Cat. bs‐23382R); Anti‐SCARB1 (Bioss, Cat. bs‐23977R).

    Techniques: Staining, Quantitative RT-PCR